Compatible microRNA Purification Methods

We highly recommend the Qiagen miRNeasy product line of kits for cells, tissue, serum, plasma, and FFPE tissues.

Trizol extraction also works well. For Trizol purifications, glycogen does not interfere with the assay. We recommend precipitating with ethanol.

The Norgen BioTek kit works but gives 3-5 fold lower signal than Qiagen and is therefore not recommended for low RNA content samples.

Qiagen RNeasy Total RNA extraction kits preferentially select for RNAs longer than 200bp and have not been validated with our assay.

IMPORTANT NOTE: Life Technologies/Ambion miRVana kit is incompatible with our particles and cannot be used.